Birthdating of myenteric neuron subtypes in the small intestine of the mouse.

Intrinsic sensory neurons of mouse gut – towards a detailed knowledge of enteric neural circuitry across species. Focus on “Characterization of myenteric sensory neurons in the mouse small intestine”

Intrinsic sensory neurons of mouse gut--toward a detailed knowledge of enteric neural circuitry across species. Focus on "characterization of myenteric sensory neurons in the mouse small intestine".

The enteric nervous system of the gut is largely self-contained and includes intrinsic sensory (or primary afferent) neurons, a variety of interneurons and several classes of motor neurons (Bornstein et al. 2002; Furness et al. 2004a). Detailed studies of guinea pig ileum have identified all of these neurons and allowed construction of a model neural circuit that accounts for some intestinal be...

Proof-of-concept: neonatal intravenous injection of adeno-associated virus vectors results in successful transduction of myenteric and submucosal neurons in the mouse small and large intestine.

BACKGROUND Despite the success of viral vector technology in the transduction of the central nervous system in both preclinical research and gene therapy, its potential in neurogastroenterological research remains largely unexploited. This study asked whether and to what extent myenteric and submucosal neurons in the ileum and distal colon of the mouse were transduced after neonatal systemic de...

Evaluation of effect of chick embryonic notochord on neural induction of mouse embryonic stem cells

Introduction: The aim of this study was evaluate the ability of notochord to induce neural induction and/or differentiation of mouse embryonic stem cell to neuron and motor neuron, respectively. Methods: In order to produce embryoid bodies, ES cells line Royan B1 were grown in suspension in the absence of LIF for 4 days. EBs were divided into 4 groups. EBs in group 1 & 2 were further cultur...

Characterization of myenteric sensory neurons in the mouse small intestine.

We recorded from myenteric AH/Dogiel type II cells, demonstrated mechanosensitive responses, and characterized their basic properties. Recordings were obtained using the mouse longitudinal muscle myenteric plexus preparation with patch-clamp and sharp intracellular electrodes. The neurons had an action potential hump and a slow afterhyperpolarization (AHP) current. The slow AHP was carried by i...